HPLC Purification Samples Benefit from Fast Lyophilisation Technique...

HPLC Purification Samples Benefit from Fast Lyophilisation Technique...

Release Date: May 21, 2009

Genevac have developed an effective Fast Lyophilisation technique for preparing weighable samples from difficult-to-dry HPLC purification fractions. 

Using HPLC purification methods involving solvents such as water or DMSO - life science researchers regularly encounter a significant number of samples that do not dry well post purification. Typically, these stubborn samples form a gum or oil that creates problems including accelerated sample degradation (due to residual solvent) and difficulty in accurate weight determination. Using a Genevac HT Series evaporator and proprietary LyoSpeed™ technology, HPLC fractions can be concentrated to a few millilitres and then frozen and rapidly lyophilised to produce a diffuse dry powder, which can easily be re-dissolved or weighed out. 

Traditional lyophilisation is a trusted multi-stage drying process, based upon sublimation, used to extract water from pharmaceuticals, foods and natural products such that the resultant samples remain stable and are easier to store at room temperature. However, the slow speed of the traditional lyophilisation process and limitation of solvents that can be removed sometimes restrict its potential. A study done at the Genevac laboratory (http://www.genevac.org/en/ArticleDetail.asp?S=6&V=1&ProductDownload=83) shows that HPLC purification fractions containing water can be lyophilised in a greatly reduced time using LyoSpeed technology, while providing the same sample quality. 

Genevac, a SP Industries company, was founded in 1990. Today the company employs around 85 people, with manufacturing, R&D and marketing headquartered in Ipswich, UK. Genevac today offers a comprehensive portfolio of evaporators to suit almost any solvent removal application, purchasing budget or productivity requirement. 

For further information on the HT Series Centrifugal Evaporator and LyoSpeed technology please contact Genevac on +44-1473-240000 / +1-845-687-5000 or email salesinfo@genevac.co.uk.

Service Contact Information

For SP Service & Support Enquiries - Including Spare Parts

Email: service@spscientific.com

For SP Customer Service Enquiries

Email: csg@spscientific.com

Featured Product

LyoConstellation S30 Freeze Dryer
LyoConstellation Development & Pilot Freeze Dryers

LyoModelling Calculator

LMC Icon

New LyoTool to 

model a freeze-drying cycle 

Request a Quote

Interested in learning more about our products?

Our Lyo Tools

LyoTools Circles


Our Lyo Tools include the Dictionary, the Conversion Calculator and the Vapor Pressure Chart.

"The technical information on your website, and the free lyo seminars you offer are a testament to your expertise.  The resources are truly invaluable and appreciated."

Actual Client Testimonial


About Our Trusted Brands