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SP Scientific… scientific equipment you have come to know and trust - FTS Systems, Genevac, Hotpack, Hull, PennTech and and Virtis.

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LyoLearn Webinars

Free online seminar series with topics of interest to lyophilization scientists and presented by industry experts.

Live sessions allow participants to interact with renowned freeze-drying specialists, such as Dr. John Carpenter, Dr. Steve Nail, Dr. Michael Pikal, and other who's who of the freeze-drying community.

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Freeze Drying of Cells and Tissues for Applications in Regenerative and Reproductive Medicine
Date & Time: 11/28/2018 10:00 AM - 2:00 PM ET Presenter(s): Willem F. Wolkers, Prof. Dr. Ir.
View Abstract
- Session 1
  
  11/28/2018 10:00 AM - 11:00 AM ET
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- Session 2
  
  11/28/2018 1:00 PM - 2:00 PM ET
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A discussion of challenges and methodology of freeze-drying cells and tissues across applications, specifically in regenerative and reproductive medicine. View abstract for more information.

Cryopreservation and freeze drying are widely accepted as the preferred techniques for long-term storage of biological materials in medical applications. Whereas cryopreserved samples are typically stored in liquid nitrogen, dried samples can be stored at room temperature, which has clear advantages for banking and transport. Freeze-drying involves a freezing and a drying step both imposing stress. Freezing and drying may cause irreversible structural changes in proteins and phase changes in membranes. Freezing and drying distorts the membrane permeability barrier function causing leakage and uptake of membrane impermeable molecules. Non-reducing disaccharides have been shown to stabilize biomolecules during drying by forming direct hydrogen bonding interactions and by forming a glassy state. Attempts to stabilize cells in the dry state have focused on disaccharides as main stabilizing compound. The major challenge in using disaccharides for drying of cells is to overcome the impermeability of cell membranes to disaccharides, to provide intracellular protection. Freeze-drying of tissues requires protection of the delicate extracellular matrix histoarchitecture. Freeze-dried decellularized tissues can be freeze-dried and used as matrix for tissue regeneration: examples are heart valves and skin. In this presentation, feasibility of freeze-drying for stabilization of cells as well as decellularized heart valves will be discussed. Various methods to incorporate disaccharides into mammalian cells will be highlighted including a novel method making use of freezing-induced membrane phase transitions. This is illustrated for platelets, red blood cells, and sperm. Furthermore, it is shown how disaccharides can be used for freeze-drying of decellularized heart valve scaffolds. Diffusion of lyoprotective agents into the scaffolds has been studied by Fourier transform infrared spectroscopy, and the effects of freeze-drying on the overall histoarchitecture, matrix proteins, and biomechanical properties are shown.

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SP Scientific is the synergistic collection of well-known and highly regarded scientific equipment brands, including VirTis, FTS Systems, Hull, Genevac, Hotpack, and most recently PennTech. The joining of these well-established brands as SP Scientific has created one of the largest and most experienced companies in freeze drying (lyophilization), centrifugal evaporation and concentration, temperature control/thermal management, glassware washers, controlled environments, vial washing and tray loading machines.

More than a scientific equipment supplier, SP Scientific represents brands that distinguish themselves by being able to more thoroughly assist customers in matching equipment to particular application needs. Fortune 500, pharmaceutical, aerospace, automotive, medical device, diagnostic kit and biotechnology companies — as well as government facilities, universities and colleges are among the organizations served on a daily basis.

SP Scientific is constantly striving for superior service and quality. We want to ensure that our customers become part of our “family” and that we serve each customer to the best of our abilities. Each manufacturing facility is an ISO 9001:2008 rated facility. Industries such as pharmaceutical manufacturing have required that the associates at SP Scientific are well versed in current Good Manufacturing Practices (cGMP), Good Automated Manufacturing Practices (GAMP5), 21CFR, Part 11, electronic signatures and ASME standards.

SP Scientific is a global company with manufacturing facilities in Gardiner and Stone Ridge, New York; Warminster, Pennsylvania; and Ipswich, United Kingdom. Regional sales associates, Distributors and service technicians are located throughout the world. SP Scientific associates and distributors look forward to helping you reach your goals.

SP Scientific is part of SP Industries, Inc. , a leading designer and manufacturer of state-of-the-art laboratory equipment, pharmaceutical manufacturing solutions, laboratory supplies and instruments, and specialty glassware. SP's products support research and production across divers end user markets including pharmaceutical, scientific research, industrial, aeronautic, semiconductor and healthcare. SP's three flagship brands -- SP Scientific, SP Scienceware, and SP Ableware -- represent over 400 combined years of experience, quality, and innovation. In December, 2015, SP Industries was acquired by Harbour Group, a private investment firm founded in 1976. Harbour Group is a privately owned, operations focused company based in St. Louis, Missouri. Headquartered in Warminster, Pennsylvania, SP Industries has production facilities in the USA and Europe and offers a world-wide sales and service network with full product support including training and technical assistance.

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